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CliniMACS Prodigy: cell processing - Digital Recording (Nov. 13, 2013)

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ISCT Webinar: CliniMACS Prodigy - Mastering the complexity of cell processing

Sponsored by Miltenyi Biotec

Please open the PDF slide and click the link on the first page to access the Digital Recording.


Date: November 13th, 2013

Time: 12pm ET to 1:00pm ET

Chair: Hermann R. Bohnenkamp, Ph.D., Miltenyi Biotec GmbH, Bergisch Gladbach, Germany

Speaker(s): Laurence J.N. Cooper, M.D., Ph.D., MD Anderson Cancer Center, Houston, Texas, USA and Mario Assenmacher, Ph.D., Miltenyi Biotec GmbH, Bergisch Gladbach, Germany


About the Webinar: 

Since its introduction in 1997, the CliniMACS Cell Separation System has become a proven technology for the development and establishment of cell-based therapies. Now, the new CliniMACS Prodigy System provides advanced integrated solutions for streamlining cell-processing workflows: from cell separation, through cell culture, to formulation of the final product. With this flexible technology platform, fully automated cell processing for innovative and complex cell manufacturing protocols becomes a reality.Within this webinar the CliniMACS Prodigy System will be introduced and the Cytokine Capture System (IFN-gamma) process for the manufacturing of multi-antigen specific T cells will be outlined. 

Adoptive transfer of T-lymphocytes with specificities for one or several viruses is a promising strategy to treat or prevent opportunistic infections in immunocompromised individuals, e.g. in patients after allogeneic hematopoietic stem cell transplantation. In this webinar the rapid and fully automated generation of virus-specific T-cells reactive against CMV, AdV, and EBV antigens will be described. 

Additionally, the comparison of the cellular products generated by the established CliniMACS plus procedure with the CliniMACS Prodigy System will be discussed. Tri-virus-specific T-cells and its composition in respect to single antigen specificities will be phenotypically and functionally analyzed. 

Learning objectives:
Manufacturing processes for complex procedures

Introduction to the CliniMACS Prodigy Instrument

Technical challenges for manufacturing multi-antigen specific T cells for clinical use

Manufacturing of single- or multi-antigen specific T cells

Verification and comparability of CliniMACS plus and CliniMACS Prodigy Instruments

Analysis of the cellular product

Phenotypical and functional analysis platform for antigen-specific T cells

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